Question 1
Which of the following will NOT be triggered by the release of acetyl choline in the synapse at the neuromuscular junction during muscle contraction?
- A. Generation of an action potential in the muscles
- B. Release of Ca²⺠from the sarcoplasmic reticulum
- C. Binding of ATP molecules to the myosin head
- D. Shifting of tropomyosin to expose the myosin binding sites on actin filaments
Show answer and rationale
Correct answer: C — Binding of ATP molecules to the myosin head
At the neuromuscular junction, acetylcholine release initiates muscle contraction by binding to receptors on the muscle membrane, causing depolarization and an action potential that propagates along the fiber. This triggers calcium release from the sarcoplasmic reticulum, which binds to troponin, shifting tropomyosin to expose myosin-binding sites on actin, enabling contraction. These events form a direct cascade from acetylcholine's action. However, ATP binding to the myosin head occurs later in the cross-bridge cycle, detaching myosin from actin after a power stroke, a process not directly initiated by acetylcholine but part of the contraction-relaxation cycle powered by ATP hydrolysis. This distinction highlights that while calcium and actin exposure are immediate downstream effects, ATP's role is a subsequent step, reliant on energy dynamics rather than the initial synaptic signal, making it the exception in this sequence.